Hybridoma Myeloma Cell Culture Media
STEMCELL Technologies ClonaCell™-HY Medium E, Size: 500 mL
ClonaCell™-HY Medium E is a serum-containing, nutritionally rich liquid medium optimized for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. The medium contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin used during the selection process. This medium has been verified for use in mouse and rat hybridoma development and monoclonal antibody production and reportedly is compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster. ClonaCell™-HY Medium E has proven effective for subcloning and stabilization of unstable hybridoma cell lines.
STEMCELL Technologies ClonaCell™-HY Medium D
Perform hybridoma selection and cloning in 1 step with ClonaCell™-HY Medium D. Using this methylcellulose-based medium, easily isolate individual clones with a high probability of monoclonality for further screening and expansion. Hybridoma clones typically have a wide range of growth rates and productivities; when cloned using this product, they form visible and discrete monoclonal colonies, preventing the loss of rare and high-producing clones due to overgrowth. The hybridoma colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid medium for screening and expansion.
STEMCELL Technologies ClonaCell™-HY Hybridoma Kit, Size: 1 Kit
Complete kit for hybridoma generation
Sigma Aldrich Fine Chemicals Biosciences HAT Media Supplement (50×) Hybri-Max™ 10 vials
γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma.
STEMCELL Technologies ClonaCell™-HY Medium A, Size: 500 mL
ClonaCell™-HY Medium A is a serum-containing liquid medium formulated for the growth and expansion of parental myeloma cells prior to fusion and for the growth of stable hybridoma clones post-selection. This medium has been verified for use during mouse and rat hybridoma development and reportedly is compatible for production of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster.
ILEX LIFE SCIENCES LLC HYBRIDBOOST SFM 1000 ML
PAN-Biotech HybridBoost: Serum-free, defined medium for hybridoma cells, w: stable Glutamine, w: Insulin hum. rec., w/o: Phenol red, w: 2.438 g/L NaHCO3 (1000 ml). INTRO: HybridBoost is a serum-free and defined culture medium specifically designed for enhanced in vitro growth and proliferation of hybridoma cell lines at 37 °C in a humidified atmosphere with 5% CO2. The optimized formulation allows the generation of a high yield of monoclonal antibodies (mAb). Because it does not contain hydrolysates and only negligible amounts of animal-derived components (<0.1%) and proteins (<0.1%), it is ideal for large-scale production of mAb. Additionally, it includes a surfactant, eliminating the need of additional supplements for agitated suspension culture. MANUFACTURER: PAN-Biotech GmbH (Germany). STORAGE: +2-8°C. BICARBONATE: With 2.438 g/L sodium bicarbonate. CELL TYPE: Hybridoma. CULTURE TYPE: Suspension. GLUTAMINE: With Stable Glutamine. PHENOL RED: Without Phenol Red. STERILE: Yes.
ILEX LIFE SCIENCES LLC PAN-Biotech Panserin 293A, Serum-free medium for HEK-Cells in adherent culture, w: L-Glutamine (100 ml)
PAN-Biotech Panserin 293A, Serum-free medium for HEK-Cells in adherent culture, w: L-Glutamine. QUANTITY: 100 ml. DESCRIPTION: Panserin 293A is a ready-to-use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in adherent culture. Serum-containing medium can be replaced directly. Panserin 293A promotes cell attachment and high cell growth rates, and has been used for the expression of recombinant proteins and the proliferation of AVVs (Adeno-associated virus vectors) and LVVs (lentiviral vectors). The efficient serum-free cultivation can be supported by higher seeding densities. COMPOSITION: Based on DMEM, Panserin 293A contains additional trace elements, proteins, cholesterol, lipids, vitamins and hormones. The total content of proteins and animal-derived components is lower than 0.2% w/v.
ILEX LIFE SCIENCES LLC PAN-Biotech Panserin 293A, Serum-free medium for HEK-Cells in adherent culture, w: L-Glutamine (6 x 500 ml)
PAN-Biotech Panserin 293A, Serum-free medium for HEK-Cells in adherent culture, w: L-Glutamine. QUANTITY: 6 x 500 ml. DESCRIPTION: Panserin 293A is a ready-to-use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in adherent culture. Serum-containing medium can be replaced directly. Panserin 293A promotes cell attachment and high cell growth rates, and has been used for the expression of recombinant proteins and the proliferation of AVVs (Adeno-associated virus vectors) and LVVs (lentiviral vectors). The efficient serum-free cultivation can be supported by higher seeding densities. COMPOSITION: Based on DMEM, Panserin 293A contains additional trace elements, proteins, cholesterol, lipids, vitamins and hormones. The total content of proteins and animal-derived components is lower than 0.2% w/v.
ILEX LIFE SCIENCES LLC PAN-Biotech Panserin 413, Serum-free Medium for Lymphocytes, T-Cells and Hybridoma Cells, w: L-Glutamine, modified (500 ml)
PAN-Biotech Panserin 413: Serum-Free Medium for Lymphocytes, T Cells, and Hybridoma Cells, w: L-Glutamine, modified. QUANTITY: 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). DESCRIPTION: Panserin 413 is a ready-to-use, serum-free, low protein (<0.1% w/v) and low animal-derived components (< 0.6%) medium for the serum-free cultivation and proliferation of lymphocytes from whole blood. The medium can be used for the enrichment of T cells , cytokine-induced killer (CIK) cells, lymphokine-activated killer (LAK) cells, and natural killers (NK) cells. COMPOSITION: This defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. The medium contains no hydrolysates, and only traces of animal-derived components (< 0.6% w/v) and proteins (< 0.1% w/v).
ILEX LIFE SCIENCES LLC PAN-Biotech Panserin 413, Serum-free Medium for Lymphocytes, T-Cells and Hybridoma Cells, w: L-Glutamine, modified (6 x 500 ml)
PAN-Biotech Panserin 413: Serum-Free Medium for Lymphocytes, T Cells, and Hybridoma Cells, w: L-Glutamine, modified. QUANTITY: 6 x 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). DESCRIPTION: Panserin 413 is a ready-to-use, serum-free, low protein (<0.1% w/v) and low animal-derived components (< 0.6%) medium for the serum-free cultivation and proliferation of lymphocytes from whole blood. The medium can be used for the enrichment of T cells , cytokine-induced killer (CIK) cells, lymphokine-activated killer (LAK) cells, and natural killers (NK) cells. COMPOSITION: This defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. The medium contains no hydrolysates, and only traces of animal-derived components (< 0.6% w/v) and proteins (< 0.1% w/v).
ILEX LIFE SCIENCES LLC PANSERIN 413 SFM 1000 ML
PAN-Biotech Panserin 413, Serum-free Medium for Lymphocytes, T-Cells and Hybridoma Cells, w: L-Glutamine, modified (1000 ml). MANUFACTURER: PAN-Biotech GmbH (Germany). INTRO: Panserin 413 is a ready-to-use, serum-free, low protein (<0.1%) and low animal-derived components (<0.6%) medium for serum-free cultivation and proliferation of lymphocytes from whole blood. It enables expansion of T-Cells from PBMC/whole blood after stimulation (e.g. with PHA-L or anti-CD3), expansion of NK, LAK, and CIK-cells after stimulation, and long-term cultivation of immortalized T-cells. APPLICATION: Suspension culture of T cells, CIK cells, LAK cells, or NK cells. STORAGE TEMP: +2-8°C. COMPOSITION: Defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. REAGENTS NOT INCLUDED: Mitogens (ex. PHA-L), or antibodies (ex. CD3+), are necessary for cell differentiation or enrichment of T-cells from whole blood/PBMC.
ILEX LIFE SCIENCES LLC PANSERIN 413 SFM 20 X 500 ML
PAN-Biotech Panserin 413, Serum-free Medium for Lymphocytes, T-Cells and Hybridoma Cells, w: L-Glutamine, modified (20 x 500 ml). MANUFACTURER: PAN-Biotech GmbH (Germany). INTRO: Panserin 413 is a ready-to-use, serum-free, low protein (<0.1%) and low animal-derived components (<0.6%) medium for serum-free cultivation and proliferation of lymphocytes from whole blood. It enables expansion of T-Cells from PBMC/whole blood after stimulation (e.g. with PHA-L or anti-CD3), expansion of NK, LAK, and CIK-cells after stimulation, and long-term cultivation of immortalized T-cells. APPLICATION: Suspension culture of T cells, CIK cells, LAK cells, or NK cells. STORAGE TEMP: +2-8°C. COMPOSITION: Defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. REAGENTS NOT INCLUDED: Mitogens (ex. PHA-L), or antibodies (ex. CD3+), are necessary for cell differentiation or enrichment of T-cells from whole blood/PBMC.
ILEX LIFE SCIENCES LLC PAN-Biotech Panserin 293S, Serum-free medium for HEK-Cells in suspension culture, w: L-Glutamine (20 x 500 ml)
PAN-Biotech Panserin 293S: Serum-free medium for HEK293 cells in suspension culture, w: L-Glutamine. QUANTITY: 20 x 500 ml. MANUFACTURED BY: Pan-Biotech (Germany). DESCRIPTION: PAN-Biotech Panserin 293S is a complete, ready to use medium for the serum-free cultivation of HEK293 cells (Human Embryonic Kidney) in suspension (non-adherent) culture. COMPOSITION: Based on DMEM/F12 medium with additional oligopeptides (hydrolysates < 0.02% w/v), hormones and trace elements to support expression and cell growth. This product contains only traces of protein (< 0.002% w/v) and animal derived components (< 0.0003% w/v). APPLICATION: Panserin 293S is a particularly enriched medium optimized for the growth of HEK293 cells in suspension culture. Due to its defined composition, the purification of final products (recombinant proteins, viruses) from the cell culture is more convenient and economic. This product can also be applied to adapt adherent HEK293 cells into suspension.
ILEX LIFE SCIENCES LLC PAN-Biotech Panserin 413, Serum-free Medium for Lymphocytes, T-Cells and Hybridoma Cells, w: L-Glutamine, modified (20 x 1000 ml)
PAN-Biotech Panserin 413: Serum-Free Medium for Lymphocytes, T Cells, and Hybridoma Cells, w: L-Glutamine, modified. QUANTITY: 20 x 1000 ml. MANUFACTURED BY: Pan-Biotech (Germany). DESCRIPTION: Panserin 413 is a ready-to-use, serum-free, low protein (<0.1% w/v) and low animal-derived components (< 0.6%) medium for the serum-free cultivation and proliferation of lymphocytes from whole blood. The medium can be used for the enrichment of T cells , cytokine-induced killer (CIK) cells, lymphokine-activated killer (LAK) cells, and natural killers (NK) cells. COMPOSITION: This defined formulation based on RPMI 1640 and DMEM/F12 is optimized with additional salts, trace elements, albumin, cholesterol, lipids and vitamins. The medium contains no hydrolysates, and only traces of animal-derived components (< 0.6% w/v) and proteins (< 0.1% w/v).